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Thread: EC- PCR Testing (Polymerase Chain Reaction Testing)

  1. #1
    Wise Old Thumper
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    Default EC- PCR Testing (Polymerase Chain Reaction Testing)

    I thought EC spores where only shed in the urine via the Kidneys. So why would a Vetlab request a Urine Sample or a Faecal Sample for PCR testing for EC ?
    I AM NOW POSTING AS 'InspectorMorse' BECAUSE A TECHNICAL PROBLEM HAS LOCKED ME OUT OF MY 'Jack's-Jane' ACCOUNT ON SOME DEVICES !!

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    Wise Old Thumper SarahP's Avatar
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    How strange!
    Sarah.

    RIP Dusty and Clover bunnies xxx
    Pearl, Bramble and Hazel guinea pigs

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    Warren Scout Shel's Avatar
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    Quote Originally Posted by Jack's-Jane View Post
    I thought EC spores where only shed in the urine via the Kidneys. So why would a Vetlab request a Urine Sample or a Faecal Sample for PCR testing for EC ?
    I may come accross quite sad here and apologies if you are already aware of this, but PCR is a way of finding VERY small amounts of DNA in a sample. Could it be a way of detecting EC even if it is at the earliest stages of infection?

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    Quote Originally Posted by Shel View Post
    I may come accross quite sad here and apologies if you are already aware of this, but PCR is a way of finding VERY small amounts of DNA in a sample. Could it be a way of detecting EC even if it is at the earliest stages of infection?
    No idea, its apparently to check for 'active shedding of spores'
    I AM NOW POSTING AS 'InspectorMorse' BECAUSE A TECHNICAL PROBLEM HAS LOCKED ME OUT OF MY 'Jack's-Jane' ACCOUNT ON SOME DEVICES !!

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    Warren Scout Shel's Avatar
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    Quote Originally Posted by Jack's-Jane View Post
    No idea, its apparently to check for 'active shedding of spores'
    It sounds feasible if a little odd. Maybe they have found new methods for detecting EC and your lab is at the cutting edge After all antibody detection is not always very reliable.

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    Wise Old Thumper Hugo's There's Avatar
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    It says here that spores can be shed in faeces

    http://www.palsvetlab.co.uk/sitebuil...bitleaflet.pdf

  7. #7
    Alpha Buck
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    When PALS released that test I was also non-plussed. I discussed it with other bunny vets and we agreed that faecal testing would be less accurate as shedding is not well understood and urine testing was far preferable as we know how the parasite's life cycle progresses in the kidneys and had a better idea of how test results would fit with clinical disease.
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    www.manorvets.co.uk/exotic-pets

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    Quote Originally Posted by marie_kubiak View Post
    When PALS released that test I was also non-plussed. I discussed it with other bunny vets and we agreed that faecal testing would be less accurate as shedding is not well understood and urine testing was far preferable as we know how the parasite's life cycle progresses in the kidneys and had a better idea of how test results would fit with clinical disease.
    Thanks Marie. That's what I was thinking too.
    Not that I know much !!
    I AM NOW POSTING AS 'InspectorMorse' BECAUSE A TECHNICAL PROBLEM HAS LOCKED ME OUT OF MY 'Jack's-Jane' ACCOUNT ON SOME DEVICES !!

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